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p2、p10、p20、p100、p200、p1000、p5000、p10ml体积 准确度(平均误差) 准确度(重现性) ul 误差(ul) 误差(%) s.d.(ul) r.s.d.(ul) p2 0.2 ±0.024 ±12 ≤0.012 ≤6.00 0.5 ±0.025 ±5.0 ≤0.012 ≤2.50 2 ±0.030 ±1.5 ≤0.014 ≤0.70 p10 1 ±0.025 ±2.5 ≤0.012 ≤1.25 5 ±0.075 ±2.5 ≤0.030 ≤0.60 10 ±0.100 ±2.5 ≤0.040 ≤0.40 p5000 1000 ±12 ±1.2 ≤3 ≤0.30 2000±12 ±0.6 ≤5 ≤0.25 5000 ±30 ±0.6 ≤8 ≤0.16 p10ml 1000 ±30 ±3.0 ≤6≤0.60 2000 ±30 ±1.5 ≤6 ≤0.30 5000 ±40 ±0.8 ≤10 ≤0.20 10000 ±60 ±0.6 ≤16 ≤0.16 货号 型号 价格(rmb) f144801 pipetman p2 2340 f144802 pipetman p10 2340 f123603 pipetman p5000 2340 f161201 pipetman p10ml 2340 体积 准确度(平均误差) 准确度(重现性) ul 误差(ul) 误差(%) s.d.(ul) r.s.d.(ul) p20 2 ±0.1 ±5.0 ≤0.03 ≤1.5 50 ±0.1 ±2.0 ≤0.04 ≤0.80 10 ±0.1 ±1.0 ≤0.05 ≤0.50 20 ±0.2 ±1.0 ≤0.06 ≤0.30 p100 20 ±0.35 ±1.8 ≤0.10 ≤0.5 50 ±0.40 ±0.8 ≤0.12 ≤0.24 100 ±0.80 ±0.8 ≤0.15 ≤0.15 p200 50 ±0.5 ±1.0 ≤0.20 ≤0.40 100 ±0.8 ±0.8 ≤0.25 ≤0.25 200 ±1.6 ±0.8 ≤0.30 ≤0.15 p1000 200 ±3 ±1.5 ≤0.60 ≤1.30 500 ±4 ±0.8 ≤1.0 ≤0.20 1000 ±8 ±0.8 ≤1.5 ≤0.15 m10,m25,m50,m100,m250,m10001800/支125-0095 aminex hpx-87c column, 300 x 7.8 mm high fructose corn syrup 9 calcium 8 5–9125-0094 aminex hpx-87c column, 250 x 4.0 mm sugar alcohols (usp) 9 calcium 8 5–9125-0098 aminex hpx-87p column, 300 x 7.8 mm pentose sugars, cellulose hydrolysates 9 lead 8 5–9125-0140 aminex hpx-87h column, 300 x 7.8 mm sugars with organic acids 9 hydrogen 8 1–3125-0096 aminex hpx-42c column, 300 x 7.8 mm oligosaccharides, thickening agents 25 calcium 4 5–9125-0097 aminex hpx-42a column, 300 x 7.8 mm oligosaccharides to d-11 25 silver 4 6–8125-0105 fast carbohydrate column, 100 x 7.8 mm glucose, galactose, sucrose, fructose 9 lead 8 5–9目录 # 描述125-0094 aminex hpx-87c column, 250 x 4.0 mm 125-0095 aminex hpx-87c column, 300 x 7.8 mm 125-0096 aminex hpx-42c column, 300 x 7.8 mm 125-0097 aminex hpx-42a column, 300 x 7.8 mm 125-0098 aminex hpx-87p column, 300 x 7.8 mm 125-0105 fast carbohydrate column, 100 x 7.8 mm 125-0140 aminex hpx-87h column, 300 x 7.8 mm 125-0142 aminex hpx-87k column, 300 x 7.8 mm 125-0143 aminex hpx-87n column, 300 x 7.8 mm 1327 型记录仪是一款设计精巧的双笔记录仪,为空间局促的实验室和冷 库的理想选择。它能与许多检测装置兼容。与econo 蠕动泵、biologic lp、 或biologic duoflow 系统连接时,能自动完成记录笔的起放,走纸和操作 标记等功能。主要特征包括:• 双通道,双笔配置 • 彩色记录装置,与econo 蠕动泵, biologic lp 系统和biologic duoflow系统匹配 • 1 mv–20 v 电压范围,适合任何紫外/可见、ph、折光率或电导率检测器 • 机械的刻度偏置管,对记录笔到达到纸顶时非常有用 • 12 种走纸速率,以秒、分、小时或天为单位记录数据 目录 # 描述model 1327 dual-pen chart recorder 731-8250 model 1327 chart recorder, with usa, canada, japan, mexico, taiwan, and latin america power adaptor, includes red pen, blue pen, 2 rolls chart paper, 2 sets banana plug cables, spare fuse 731-8253 model 1327 chart recorder, with uk, commonwealth power adaptor 731-8255 model 1327 chart recorder, with european power adaptor power adaptors 731-8194 power adaptor, for model 1327 chart recorder, usa, canada, japan, mexico, taiwan, latin america 731-8195 power adaptor, for model 1327 chart recorder, uk, commonwealth (except canada, australia, and new zealand) 731-8197 power adaptor, for model 1327 chart recorder, for europe (except uk) and other countries (except usa, canada, japan, mexico, taiwan, latin america, australia, new zealand) recorder accessories 167-0220 disposable fiber tip blue short pens, for model 1327 and 1325 recorders, 3, lifetime approximay 1,000 m 167-0221 disposable fiber tip red long pens, 3, lifetime approximay 1,000 m 731-8198 chart paper, for model 1327 and 1325 recorders, 2 rolls, 10 cm x 13 m 731-8262 system cable 2, 8-pin mini-din to 8-pin standard din 731-8264 system cable 4, 8-pin mini-din to banana plug cable 731-9009 system cable 23, y-cable connecting econo gradient pump to biofrac fraction collector and model 1327 chart recorder 731-9010 system cable 22, y-cable connecting econo gradient pump to model 2110 fraction collector and model 1327 chart recorder 操作方便的2110 型组分收集器提供了多种层析分离 的收集模式。主要特性包括:• 时间收集模式或液滴收集模式(或体积收集模式—与ep–1 型econo 泵,biologic lp 或biologic hr 系统连接) • 收集1 滴(~50μl)至9 ml 体积的组分至试管或微孔板(使用转换接头) • 小型( econo–column ) 层析柱可被安装于臂上,减少死体积 • 可人工更换试管 • 可置于冷库 • 24 x 33 cm 的小底座 目录 # 描述model 2110 fraction collector 731-8120 model 2110 fraction collector, 220/240 v 731-8122 model 2110 fraction collector, 100/120 v accessories 731-8130 carousel, 80-tube capacity 731-8131 replacement dr-op formers, 2 731-8135 micro tube adaptor 731-8136 instrument dust cover 731-8261 system cable 1, 8-pin mini-din to db-9 connector 731-8265 system cable 5, db-9 connector to bare wires 731-9010 system cable 22, y-cable connecting econo gradient pump to model 2110 fraction collector and model 1327 chart recorder tubes 223-9500 micro test tubes, capless, 1.5 ml, polypropylene, natural, graduated, 500 223-9750 clear polystyrene tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000 223-9751 natural polypropylene tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000 组分收集器是分析型和制备型层析的理想选择,它提供了 完善的收集控制选择。由于编程简便,biofrac 组分收集器可 用于<100 ml/min 流速下一般或复杂的组分收集。biofrac 组分收集器既能作为单独的收集器,也可成为bio–rad biologic duoflow 和biologic lp 层析系统的理想匹 配,能提供准确可靠的组分收集。biofrac 允许灵活地使 用多种收集层析架,包括用户收集架或独立层析架。用户 收集架的收集能力从一液滴到数升不等。独立层析架提高了 收集流程的灵活性,同时提供经济的样品储存功能。独立层 析架可高压灭菌,安装简便,平稳,节省空间。组分收集器主 要特性包括:• 时间收集模式或点滴收集模式(或体积收集模式—与 biologic duoflow 系统,biologic lp 系统,或ep–1 型 econo 泵连接) • 通过峰值检测来收集产品峰,或用时间窗口(zui多20 个)收集 产品峰,或两者结合 • 液滴臂以x 轴,y 轴方式移动;对微孔板或titertube 试管提供行或列的模式选择 • 可人工调节分装臂至试管高度< 150 mm • 多种独立层析架,与试管(直径12–20 mm)、eppendorf 管或其它离心管(0.5, 1.5, 或2.0 ml)或液闪 瓶匹配 • 多达20个备用收集接头,可以将组分收集到玻璃瓶、窄口试剂瓶等多种容器中 • 带有试管夹的备用冰浴/微孔板支架,可将试管固定在任何位置;冰浴支架可固定13 mm 试管,也可作为微 孔板与titertube 试管(符合微孔板sbs 标准)的支架 • 多次运行特性,可实现组分叠加 • 性能安全,可靠,噪音低 • 微孔板收集接头能精确地将微量样品收集至微孔板中 灵活的架板选择。冰浴/微孔板支架用于 把组分收集到(a)预冷试管或(b)微 孔板,zui多4 块(图中使用了(c)25μl 接头)(d)独立层析架,增加储存能力。 备用转换接头支架(e)可将制备型组分 收集至1–20 个任何规格的容器中。biofrac 收组分集器(f)zui多能支持4 个h4–high 层析架。目录 # 描述biofrac fraction collector 741-0002 biofrac fraction collector, input voltage 100–240 v, includes power cord, rack set f1 (2 x flatpack, 13 mm), biofrac diverter valve, fittings kit, instructions accessories 741-0007 biofrac fraction collector fittings kit, includes replacement fittings and tubing for setup of the fraction collector to the biologic lp or duoflow chromotography systems 741-0010 rack set f1, 2 x flatpack, with numbered tube positions, each holds 90 tubes, 12–13 mm diameter, for total of up to 180-tube collection 741-0011 rack set f2, 2 x flatpack, each holds 60 tubes, 15–16 mm diameter, for total of up to 120-tube collection 741-0012 rack set f3, 2 x flatpack, each holds 40 tubes, 18–20 mm diameter, for total of up to 80-tube collection 741-0013 rack set h1, 4 x flatpack, each holds 42 capless 1.5 ml eppendorf/microtubes for total of up to 168-microtube collection 741-0014 rack set h2, 4 x flatpack, each holds 63 capless 0.5 ml eppendorf/microtubes for total of up to 252-microtube collection 741-0015 rack set h3, 4 x flatpack, each holds 30 reduced volume scintillation vials, 16 mm diameter, for total of up to 120-vial collection 741-0016 rack set h4, 4 x flatpack, each holds 6 scintillation vials, 30 mm diameter, for total of 24-vial collection 741-0017 biofrac ice bath/microplate rack, holds 120 tubes, 12–13 mm diameter; with the following capabilities: up to 4 sbs-format microplates in 96-, 48-, 24-, or 12-well configurations; titertube microtube collection, 8 x 12, 96-tube configuration 741-0018 biofrac prep-20 preparative rack 741-0020 biofrac h4-high rack set, 4 x flatpack, each holds 6 centrifuge tubes, 30 mm diameter, for total of up to 24-vial collection 731-8286 system cable 15, 15-pin d to mini-din 731-8287 system cable 16, 8-pin mini-din to 8-pin standard din 731-8290 biofrac accessory cable, 15-pin d to bare wires, for connecting biofrac fraction collector to other equipment; for input or output signals 731-9009 system cable 23, y-cable connecting econo gradient pump to biofrac fraction collector and model 1327 chart recorder 741-0088 biofrac microplate dr-op head kit, includes preassembled dr-ophead nut with 0.020" id tefzel tubing; delivers approximay 25 µl per dr-op tubes 223-9500 micro test tubes, capless, 1.5 ml, polypropylene, natural, graduated, 500 223-9750 clear polystyrene tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000 223-9751 natural polypropylene tubes, 13 x 100 mm, 9 ml nominal capacity, 1,000 电导率监控是优化蛋白纯化工艺和确定层析分离效果所 必需的。eg–1 型econo 梯度检测器能轻松地进行梯度检 测和电导率确定。特性包括:• 可灵活性放置的外置流动池 • 实时检测电导率(以siemens 为单位) • 梯度检测量程可调 • 可置于冷库 • 易于连接的luer–lok 接头 • 监控器可通过1/4–28 平底螺纹接头与fplc 和hplc系统连接 目录 # 描述model eg-1 econo gradient monitor 731-8150 model eg-1 econo gradient monitor, includes flow cell, system cable 4; does not include power adaptor 731-8151 model eg-1 econo gradient monitor, includes 100/120 v usa power adaptor, flow cell, and system cable 4 accessories 731-8155 conductivity flow cell 731-8270 power adaptor, 100/120 v, for usa, canada, japan, mexico, taiwan, and latin america 731-8271 power adaptor, 220/240 v, for europe (except the uk) and other countries (except usa, canada, japan, mexico, taiwan, latin america, australia, new zealand) 731-8272 power adaptor, 220/240 v, for uk, australia, and new zealand 双波长(254/280 nm)em–1 型econo 紫外检测 器,可检测层析柱的流出液。检测器由一个控制元件, 一个含254 和280 nm 滤光片的光学模块,以及一个 2 毫米长的流动池组成。通过易于更换的luer–lok 接头连接。主要特性包括:• 便携式的光学检测装置,探测器置于层析柱出口附件,以获得*的分辨率 • 内置滤光片 • 自动调零功能 • 可置于冷库 • 光电二极管显示吸收率 目录 # 描述model em-1 econo monitor 731-8154 model eg-1 econo gradient monitor, includes 100/120 v (us) power adaptor, flow cell, system cable 4 731-8160 model em-1 econo uv monitor, 110/120 v, includes control module, optics module, filters for 254 and 280 nm wavelengths, system cable 4, fittings kit 731-8162 model em-1 econo uv monitor, 220/240 v accessories 731-8165 uv flow cell, replacement 731-8166 lamp, replacement 731-8167 filter assembly, 280 and 254 nm 731-8168 model em-1 optics module assembly ep–1 型econo 泵是一个速度可调、双通道双向 蠕动泵,用于低压层析或在常规实验室操作中抽吸液 体。它具有独立蠕动泵的所有特性,并与biologic duoflow 系统*匹配。主要特性包括:• 流速显示(ml/min)及时间和体积控制操作程序 • 预设标准管线尺寸(0.8, 1.6 和3.2 mm id)校正 • 非标准管线尺寸或更高流速精度手动校正 • 控制2110 型、biofrac 或其它组分收集器 • 通过程序化的运行总体积来控制运转周期 • 控制sv–3 螺线管分流阀,把流出液分流至收集器或废液槽 目录 # 描述model ep-1 econo pump 731-8140 model ep-1 econo pump, 100/120 v 731-8142 model ep-1 econo pump, 220/240 v cables 731-8261 system cable 1, 8-pin mini-din to db-9 connector 731-8263 system cable 3, 8-pin mini-din to 8-pin mini-din 731-8267 system cable 7, 8-pin mini-din to bare wires 731-8286 system cable 15, 15-pin d to mini-din • 可与1327 型记录仪连接使用 *的econo 梯度泵适用于任何低压蛋白纯化系统。它是*能独立应用于均一洗脱和 梯度洗脱的蠕动泵。econo 梯度泵是一个双通道双向泵,应用于制备层析或重组蛋白亲和 层析纯化。econo 梯度泵可与2110 型、biofrac 组分收集器,em–1 型econo 紫外检 测器、eg–1 型econo 梯度检测器和1327 型记录仪等设备*匹配。该econo 梯度 组合泵是您经济而实用的*。开发新的应用领域• 串联收集— 将层析柱流出液分流,收集至两个不同部位的试管或微孔板中,作进一部的测试和分析 • 取样操作— 发酵罐在发酵进程中可例行取样,以监控目标产品的浓度;培养液通过econo 梯度泵再循环,分流阀定时将等量试样送至组分收集器作进一步分析 • 实时生物活性检测— 流出液中添加底物来检测反应混合物。econo 梯度泵可提供一定流速的底物,并通过三 通管与分装的流出液混合。收集反应混合液作进一步 分析,或送至流动池记录生色团的变化 梯度泵具有以下优点:• 每个通道的流速0.002–40ml/min (每泵2 个通道,zui高流速达20ml/min );分装阀组合套件流速可达> 2μl/min • 控制二元梯度混合器 • 安装、操作简便 • 时间和体积两种梯度控制模式 • 控制分流阀收集废物或组分(尤其适合较大体积的分子量排阻层析) • 控制组分收集器的启动/停止;一些组分收集器具有“等待”和“暂停”功能 • 控制记录仪的启动/停止,记录笔的起/落 • 控制分装阀的百分比增量 • 根据不同规格管线自动调节收集程序 • 经济、简便、快速连接的luer–lok 接头 • 液晶显示器对比度可调,*的记数器确认管线磨损程度,有效按键蜂鸣声 • 节省空间的整合设计 • 与biologic duoflow 层析系统兼容 目录 # 描述econo gradient pump 731-9001 econo gradient pump, 100/120 v, includes tubing and fittings kit 731-9002 econo gradient pump, 220/240 v, iincludes tubing and fittings kit combination systems 731-9030 econo gradient pump combo 1, 100/120 v, includes econo gradient pump, gradient mixer valve 731-9032 econo gradient pump combo 1, 220/240 v, includes econo gradient pump, gradient mixer valve 731-9034 econo gradient pump combo 2, 100/120 v, includes econo gradient pump, gradient mixer valve, mv-6 injection valve, rack with column clamps 731-9036 econo gradient pump combo 2, 220/240 v, includes econo gradient pump, gradient mixer valve, mv-6 injection valve, rack with column clamps 731-9038 econo gradient pump combo 3, 100/120 v, includes econo gradient pump, gradient mixer valve, rack with column clamps 731-9040 econo gradient pump combo 3, 220/240 v, includes econo gradient pump, gradient mixer valve, rack with column clamps accessories 731-9004 econo gradient pump rack, preassembled 731-9006 econo gradient pump fittings kit, includes 32 fittings, 12 tubing retainers 731-9007 econo gradient pump tubing kit, includes 2 each of 0.8, 1.6, and 3.2 mm pharmed tubing cables 731-9009 system cable 23, y-cable connecting econo gradient pump to biofrac fraction collector and model 1327 chart recorder 731-9010 system cable 22, y-cable connecting econo gradient pump to model 2110 fraction collector and model 1327 chart recorder valves 731-8320 mv-6 sample inject valve, 6 ports 731-8322 sv-3 diverter/bypass valve, 3-way valve 731-8323 gradient mixer 731-9011 splitter valve kit, includes valve, tubing, adaptors, t-union, backpressure device 液流选择和自动加样阀sv–5 液流选择阀拓展了biologic lp 系统制备纯化的性能,可选择性控制5 种缓冲液和自动输入大体积样品。 sv–5 阀直接安置于biologic 层析架上,由biologic lp 系统调控。人工进样阀mv–6 进样阀有6 个带有凹 luer 接头的口。它能与任何容积的自制上样环匹配。mv–6 阀直接安置于biologic 系统层析架和econo 梯度泵支架上。组分收集/层析柱旁通阀sv–3 分流/旁通阀是由econo 梯度泵、ep–1 型econo 泵或biologic lp 系统控制的二相螺线管阀。当与 biologic lp 系统连接时,sv–3 阀将液体从层析柱导入 至旁通位点,或至组分收集器或至废液位点。阀的功 能由后置的mini–din 接头和阀管线决定。当与 econo 泵或econo 梯度泵连接,sv–3 阀只作为组分收集器分流 阀使用。sv–3 阀直接与biologic 系统层析架和econo 梯度泵支架连接。 biologic 层析架是一款坚固耐用、可调节的支架系统,由耐腐蚀聚丙烯、 不锈钢和玻璃尼龙等材料组成。层析架既可单独放置又可与任何biologic duoflow 或biologic lp 系统结合,节省实验空间。它可支持一系列的层析 柱、滤柱、阀、检测器、试剂瓶以及诸如2110 型组分收集器和1327 型记录 仪等外围设备。我们提供biologic 层析架组合套件和支架零部件以满足用户 对不同的需求。biologic 层析架组合套件体积34 x 41 x 64 cm (w x d x h).目录 # 描述750-0251 biologic rack, includes rack tray, 8 sleeves, 2 short vertical bars, 2 long vertical bars, column clamp set, 5 bar clamps, 4 cable organizers 750-0260 column clamp set, includes 1 column clamp assembly 750-0261 biologic rack tray, includes 1 rack tray, 8 bar sleeves 750-0262 vertical bars, long, 64 cm, 2 750-0263 vertical bars, short, 10 cm, 2 750-0264 horizontal bar kit, includes 2 tie bars, 4 bar clamps 750-0265 bar clamps, 5 750-0268 biologic rack expansion kit, includes 2 rack trays, 2 long vertical bars, 16 sleeves biologic duoflow 工作站在3,500 psi(233 bar,23 mpa),10ml/min 条件下,可与f10 泵 配套使用;在1,000 psi(66 bar,6.6 mpa), 40ml/min 条件下可与f40 泵配套使用。每个泵头 的流速通过添加maximizer 阀系统 而加倍。f10 和f40 泵组合套件能使您方便地选 择需要的泵头,增加biologic duoflow 系统性能。 组合套件包括有密封圈和止回阀的安装泵头,可 快速、简便地更换泵头。目录 # 描述biologic duoflow mixers 760-2010 biologic maximizer mixer 760-0170 model mx-1 mixer, includes mixer body (263 µl) and standard mixer barrel for total volume of 750 µl 760-0171 mixer barrel extender, for total volume of 2 ml; one included in the f40 pump kit (760-0180) pump kits 760-0110 f10 pump kit, converts f40 workstation to f10 pumps to enable flow rates as low as 0.01 ml/min; includes 2 fully assembled pumpheads, 4 piston assemblies, f10 tubing kit, tools, instructions 760-0180 f40 pump kit, expands pumping capabilities to 40 ml/min; includes 2 fully assembled pumpheads with seals and check valves installed, 4 piston assemblies, mixer barrel extender, 2 mm uv flow cell, f40 tubing kit, tools, instructions ezlogic积分软件同biologic duoflow软件 具有一样简洁的图形界面,并在后者基础上加入了定 量分析功能。您能通过使用指南在zui短的时间内熟悉 相关的分析术语。这个功能强大的软件包能符合您的 所有工作需要,如样品定量、层析图的积分和叠加、 确定保留时间、观察结果和制作个性化的研究报告。 图形功能包括分屏观察层析图、缩放、多参数注释和 色彩选择等。目录 # 描述750-0111 ezlogic integration software package 内置式duoflow ph 检测器可对运行中的样品ph 进行实时监控。该检测器通过一个bnc 接头与所有biologic duoflow maximizer 和biologic duoflow pathfinder 系统连接。也可通过信号输入调制器(sim)与biologic duoflow 和biologic hr 系统连接。ph 检测器同时能提供1/4–28 接头的管线组合套件。其主要性能包括:• 高精度的甘汞电极,与所有缓冲液兼容(而ag/agcl 电极与tris 缓冲液不兼容) • 参考电极和ph 电极一体整合,无需添加电解液防止参考电极干涸 • 80ml/min流速 目录 # 描述760-2040 biologic duoflow ph monitor, includes sim module, ph electrode, flow cell, and tubing 760-2042 ph electrode 760-2044 flow cell 760-2046 ph tubing kit, includes orange and green peek 1/4–28 prefitted tubing lengths for connection of the ph flow cell to the chromatography system • 80μl 扫描体积的生物兼容peek 流动池,产生高精度和高准确度的检测 maximizer 阀系统拓展了biologic duoflow 系统 的功能。模块式的maximixer 组件能提供缓冲液在线配 制、倍增流速和标准duoflow 系统双倍的阀能力。 maximixer 可以作为任何现成duoflow 系统的添加组 件,同时也可以作为biologic maximizer 和biologic duoflow pathfinder 系统的一部分。 maximizer 为biologic duoflow 系统增加了以下功能:• 从储存液中配制特定ph 和盐浓度的缓冲液 • 自动控制ph,运行多组不同ph 缓冲液或盐浓度过程 • biologic duoflow 4.0 或更高版本的软件提供层析图比较功能,能提供无* 的层析图叠加,利于工艺优化 • 增加流速,可运行大规模柱层析研究所需的大层析柱 • 控制阀(6 个低压和6 个高压)能提供更多样化的样品处理能力 • 12ml 大型动态混合器能确保80ml/min 流速 biologic duoflow pathfinder 系统包含了maximizer 阀系统目录 # 描述760-2200 biologic maximizer kit, 100/120 v, for us, japan, korea; includes maximizer base unit, ph monitor, maximizer mixer, starter kit, tubing kit, system cable 30, us power cord 760-2204 biologic maximizer kit, 220/240 v, includes maximizer base unit, ph monitor, maximizer mixer, starter kit, tubing kit, system cable 30 760-2220 biologic duoflow software, version 4.0 upgrade kit, includes biologic duoflow software cd, windows 2000 software, usb bitbus communicator, system cables 30 and 31 (bus communication and usb), instructions * maximizer upgrades require biologic duoflow version 4.0 (or higher) software. the duoflow workstation may require a firmware upgrade; for more information, please contact your local bio-rad representative. biologic duoflow 层析系统不但可灵活地与多种系统相匹配,而且提供了多种升级的选择,并有一个直观、易操作的通用控制软件。根据需要为系统升级以增添功能biologic duoflow 模块式的组件让用户可以根据实验室空间和使用需要,设计配置自己的生物层析系统。它 们可以用在实验工作台上或冷库中, 当实验要求改变时,将系统简单地重组并升级以增添新的功能,如提高流速、 完善检测功能、ph 监控、层析图比较和缓冲液自动配制等功能。一些系统的功能以及可选择的升级方案将在下页的biologic duoflow 系统选择指南中进行介绍。biologic duoflow pathfinder 层析系统(图中所示包括附加的avr9–8 和sv5–4 阀),可简便地应用于多种层析技术操作,包括基本实验步 骤操作以及新方法新工艺的优化。biologic duoflow 系统选择指南系统组件所在的页 106 106 124 110 109 113 106–107biologic duoflow 基础系统3,500 psi 10 ml/min 流速254/280 nm 波长检测 标准 可升级 可升级 可升级 可升级 可升级 可升级biologic duoflow 标准系统3,500 psi 10 ml/min 流速254/280 nm 波长检测组分收集器 标准 可升级 标准 可升级 可升级 可升级 可升级biologic duoflow quadtec 基础系统3,500 psi 10 ml/min 流速4 波长同步检测 标准 可升级 可升级 标准 可升级 可升级 -biologic duoflow quadtec 标准系统3,500 psi 10 ml/min 流速4 波长同步检测组分收集器 标准 可升级 标准 标准 可升级 可升级 -biologic duoflow maximizer 20 系统3,500 psi 20 ml/min 流速254/280 nm 波长检测缓冲液配制ph 检测组分收集器 标准 可升级 标准 可升级 标准 标准 可升级biologic duoflow maximizer 80 系统1,000 psi 80 ml/min 流速254/280 nm 波长检测缓冲液配制ph 检测组分收集器 可升级 标准 标准 可升级 标准 标准 可升级biologic duoflow pathfinder 20 系统3,500 psi 20 ml/min 流速4 波长同步检测缓冲液配制ph 检测组分收集器 标准 可升级 标准 标准 标准 标准 -biologic duoflow pathfinder 80 system1,000 psi 80 ml/min 流速4 波长同步检测缓冲液配制ph 检测组分收集器 可升级 标准 标准 标准 标准 标准 -更多信息,见106-125 页,或索取资料# 2687a。bio–rad 公司为biologic duoflow 层析系统及其外围设备, 如用于缓冲液自动配制的biologic maximizer 阀系统、biologic quadtec 紫外/可见光检测器和biofrac 组分收集器等,提供操作合格性检验规程和服务。bio–rad 公司的iq/oq 操作规程是为帮助用户遵循美国fda 认证而设计的,由经过培训、并有资格的技术人员遵循nist 标准来完成。iq 服务确保biologic duoflow 层析系统正确安装,oq 服务确保所安装层析系统的性能并提供相应的安全特性记录。目录 # 描述biologic duoflow maximizer systems 760-2236 biologic duoflow maximizer 20 system, 100/120 v, for japan and korea only, includes maximizer valve unit, maximizer mixer, ph monitor, usb bitbus communicator, dell controller, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample injection valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2237 biologic duoflow maximizer 20 system, 100/120 v, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller and monitor, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample injection valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2238 biologic duoflow maximizer 20 system, 220/240 v, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample injection valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2246 biologic duoflow maximizer 80 system, 100/120 v, for japan and korea only, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample injection valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2247 biologic duoflow maximizer 80 system, 100/120 v, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller and monitor, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample injection valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2248 biologic duoflow maximizer 80 system, 220/240 v, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample injection valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions biologic duoflow pathfinder systems 760-2256 biologic duoflow pathfinder 20 system, 100/120 v, for japan and korea only, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, system cable 25 (rs-232), conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2257 biologic duoflow pathfinder 20 system, 100/120 v, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller and monitor, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, system cable 25 (rs-232), conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2258 biologic duoflow pathfinder 20 system, 220/240 v, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, system cable 25 (rs-232), conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2266 biologic duoflow pathfinder 80 system, 100/120 v, for japan and korea only, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, system cable 25 (rs-232), conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2267 biologic duoflow pathfinder 80 system, 100/120 v, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller and monitor, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, system cable 25 (rs-232), conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions 760-2268 biologic duoflow pathfinder 80 system, 220/240 v, includes maximizer valve unit, maximizer mixer, ph monitor, dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, system cable 25 (rs-232), conductivity monitor, starter kit, uno q1 column, biofrac fraction collector, diverter valve, two f1 racks, instructions biologic duoflow quadtec systems 760-1136 biologic duoflow quadtec 10 system, 100/120 v, for japan and korea only, includes dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, instructions 760-1137 biologic duoflow quadtec 10 system, 100/120 v, includes dell controller and monitor, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, instructions 760-1138 biologic duoflow quadtec 10 system, 220/240 v, includes dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, instructions 760-1146 biologic duoflow quadtec 10 system with biofrac fraction collector, 100/120 v, for japan and korea only, includes dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-1147 biologic duoflow quadtec 10 system with biofrac fraction collector, 100/120 v, includes dell controller and monitor, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-1148 biologic duoflow quadtec 10 system with biofrac fraction collector, 220/240 v, includes dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-4136 biologic duoflow quadtec 40 system, 100/120 v, for japan and korea only, includes dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, instructions 760-4137 biologic duoflow quadtec 40 system, 100/120 v, includes dell controller and monitor, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, instructions 760-4138 biologic duoflow quadtec 40 system, 220/240 v, includes dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, instructions 760-4146 biologic duoflow quadtec 40 system with biofrac fraction collector, 100/120 v, for japan and korea only, includes dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-4147 biologic duoflow quadtec 40 system with biofrac fraction collector, 100/120 v, includes dell controller and monitor, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-4148 biologic duoflow quadtec 40 system with biofrac fraction collector, 220/240 v, includes dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, quadtec uv/vis detector with 3 mm peek flow cell, instrument control module (icm), system cables 25 and 26 (rs-232 and icm power), conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions biologic duoflow systems 760-0036 biologic duoflow 10 system, 100/120 v, for japan and korea only, includes dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, instructions 760-0037 biologic duoflow 10 system, 100/120 v, includes dell controller and monitor, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, instructions 760-0038 biologic duoflow 10 system, 220/240 v, includes dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, instructions 760-0046 biologic duoflow 10 system with biofrac fraction collector, 100/120 v, for japan and korea only, includes dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-0047 biologic duoflow 10 system with biofrac fraction collector, 100/120 v, includes dell controller and monitor, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-0048 biologic duoflow 10 system with biofrac fraction collector, 220/240 v, includes dell controller, usb bitbus communicator, f10 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-4036 biologic duoflow 40 system, 100/120 v, for japan and korea only, includes dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, instructions 760-4037 biologic duoflow 40 system, 100/120 v, includes dell controller and monitor, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, instructions 760-4038 biologic duoflow 40 system, 220/240 v, includes dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, instructions 760-4046 biologic duoflow 40 system with biofrac fraction collector, 100/120 v, for japan and korea only, includes dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-4047 biologic duoflow 40 system with biofrac fraction collector, 100/120 v, includes dell controller and monitor, dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 760-4048 biologic duoflow 40 system with biofrac fraction collector, 220/240 v, includes dell controller, usb bitbus communicator, f40 workstation, mx-1 mixer, 3-tray rack, avr7-3 sample inject valve, fittings kit, uv detector with 5 mm flow cell and 254/280 nm filters, conductivity monitor, starter kit, uno q1 column, diverter valve, two f1 racks, instructions 1327 型记录仪是一款设计精巧的双笔记录仪,为空间局促的实验室和冷 库的理想选择。它能与许多检测装置兼容。与econo 蠕动泵、biologic lp、 或biologic duoflow 系统连接时,能自动完成记录笔的起放,走纸和操作 标记等功能。主要特征包括:• 双通道,双笔配置 • 彩色记录装置,与econo 蠕动泵, biologic lp 系统和biologic duoflow系统匹配 • 1 mv–20 v 电压范围,适合任何紫外/可见、ph、折光率或电导率检测器 • 机械的刻度偏置管,对记录笔到达到纸顶时非常有用 • 12 种走纸速率,以秒、分、小时或天为单位记录数据操作方便的2110 型组分收集器提供了多种层析分离 的收集模式。主要特性包括:• 时间收集模式或液滴收集模式(或体积收集模式—与ep–1 型econo 泵,biologic lp 或biologic hr 系统连接) • 收集1 滴(~50μl)至9 ml 体积的组分至试管或微孔板(使用转换接头) • 小型( econo–column ) 层析柱可被安装于臂上,减少死体积 • 可人工更换试管 • 可置于冷库 • 24 x 33 cm 的小底座组分收集器是分析型和制备型层析的理想选择,它提供了 完善的收集控制选择。由于编程简便,biofrac 组分收集器可 用于<100 ml/min 流速下一般或复杂的组分收集。biofrac 组分收集器既能作为单独的收集器,也可成为bio–rad biologic duoflow 和biologic lp 层析系统的理想匹 配,能提供准确可靠的组分收集。biofrac 允许灵活地使 用多种收集层析架,包括用户收集架或独立层析架。用户 收集架的收集能力从一液滴到数升不等。独立层析架提高了 收集流程的灵活性,同时提供经济的样品储存功能。独立层 析架可高压灭菌,安装简便,平稳,节省空间。组分收集器主 要特性包括:• 时间收集模式或点滴收集模式(或体积收集模式—与 biologic duoflow 系统,biologic lp 系统,或ep–1 型 econo 泵连接) • 通过峰值检测来收集产品峰,或用时间窗口(zui多20 个)收集 产品峰,或两者结合 • 液滴臂以x 轴,y 轴方式移动;对微孔板或titertube 试管提供行或列的模式选择 • 可人工调节分装臂至试管高度< 150 mm • 多种独立层析架,与试管(直径12–20 mm)、eppendorf 管或其它离心管(0.5, 1.5, 或2.0 ml)或液闪 瓶匹配 • 多达20个备用收集接头,可以将组分收集到玻璃瓶、窄口试剂瓶等多种容器中 • 带有试管夹的备用冰浴/微孔板支架,可将试管固定在任何位置;冰浴支架可固定13 mm 试管,也可作为微 孔板与titertube 试管(符合微孔板sbs 标准)的支架 • 多次运行特性,可实现组分叠加 • 性能安全,可靠,噪音低 • 微孔板收集接头能精确地将微量样品收集至微孔板中 灵活的架板选择。冰浴/微孔板支架用于 把组分收集到(a)预冷试管或(b)微 孔板,zui多4 块(图中使用了(c)25μl 接头)(d)独立层析架,增加储存能力。 备用转换接头支架(e)可将制备型组分 收集至1–20 个任何规格的容器中。biofrac 收组分集器(f)zui多能支持4 个h4–high 层析架。双波长(254/280 nm)em–1 型econo 紫外检测 器,可检测层析柱的流出液。检测器由一个控制元件, 一个含254 和280 nm 滤光片的光学模块,以及一个 2 毫米长的流动池组成。通过易于更换的luer–lok 接头连接。主要特性包括:• 便携式的光学检测装置,探测器置于层析柱出口附件,以获得*的分辨率 • 内置滤光片 • 自动调零功能 • 可置于冷库 • 光电二极管显示吸收率 ep–1 型econo 泵是一个速度可调、双通道双向 蠕动泵,用于低压层析或在常规实验室操作中抽吸液 体。它具有独立蠕动泵的所有特性,并与biologic duoflow 系统*匹配。主要特性包括:• 流速显示(ml/min)及时间和体积控制操作程序 • 预设标准管线尺寸(0.8, 1.6 和3.2 mm id)校正 • 非标准管线尺寸或更高流速精度手动校正 • 控制2110 型、biofrac 或其它组分收集器 • 通过程序化的运行总体积来控制运转周期 • 控制sv–3 螺线管分流阀,把流出液分流至收集器或废液槽 • 可与1327 型记录仪连接使用biologic lp 低压层析系统是生物分子纯化的理想选择。它具有功能多、使用简便、经济等特点。小巧的设 计zui大限度地节约冷库和实验室的工作空间。biologic lp 系统出色的性能如下:• lp data view 软件— 专为biologic lp 系统设计的lp data view 软件使用简便;可完成数据采集等多重任务,只需一个串行端口即可对任何windows95, 98,me, nt4.0 或2000 操作系统的数据进行打印 • 方法程序储存— 储存50 个方法程序;每种方法包括多达50 个泵操作步骤和50 个组分收集步骤 • 缓冲液选择— 可选择5 种缓冲液,加入sv–5 液流选择阀可进行全自动样品分离和大体积样品输入 • 检测性能— 含有检测蛋白和核酸的滤光片(280nm 和254nm),以及检测梯度进程的电导检测器 • 高流速泵— 系统配置了一个流速范围0.05–40 ml/min zui大背压30 psi 的蠕动泵。系统可兼容econo–column 低压层析柱、macro–prep 层析介质、econo–pac 滤柱、amersham hitrap 滤柱、source 介质和其它所有低压层析介质 • 组份收集— 系统有简单组分和复杂组分两种收集选择: 2110 型组分收集器收集至80 根13×100 毫米试管或离心管中,或更*的biofrac 组分收集器收集至酶标板、细口大玻璃瓶等任意容器中。同时,biologiclp 系统支持许多非bio–rad 组分收集器 关于biologic lp 系统的更多信息,请索取资料# 2038 和2327。 biologic lp data view 软件操作简单的lp data view 软件可把任何能运行windows 的计算机转变成一个记录仪。但与普通的记录仪 相比,具有更为灵活的功能。它可自由调节运行中或运行后的量程,在捕获层析数据时执行多项工作,并能通 过任何或网络打印机进行打印。用户会感到瞬间储存和获取数据比以往任何时候更轻松。lp data view 软 件的功能如下:• 运行于windows 95, 98, me, nt4.0 版或2000 操作系统;需要使用一个串行端口 • 自动记录每次操作的运行信息,同时记录数据与注释 • 自动记录运行过程如启动、结束、组分收集器移动,等待,暂停和继续 • 运行数据保存于独立的数据文件 • 打印用户报告 • 数据输出 需要lp data view 软件和biologic lp 系统的演示,请与当地bio–rad 销售代 表。1327 型记录仪1327 型记录仪是一款设计精巧的双笔记录仪,为空间局促的实验室和冷 库的理想选择。它能与许多检测装置兼容。与econo 蠕动泵、biologic lp、 或biologic duoflow 系统连接时,能自动完成记录笔的起放,走纸和操作 标记等功能。主要特征包括:• 双通道,双笔配置 • 彩色记录装置,与econo 蠕动泵, biologic lp 系统和biologic duoflow系统匹配 • 1 mv–20 v 电压范围,适合任何紫外/可见、ph、折光率或电导率检测器 • 机械的刻度偏置管,对记录笔到达到纸顶时非常有用 • 12 种走纸速率,以秒、分、小时或天为单位记录数据 biologic lp 阀系统液流选择和自动加样阀sv–5 液流选择阀拓展了biologic lp 系统制备纯化的性能,可选择性控制5 种缓冲液和自动输入大体积样品。 sv–5 阀直接安置于biologic 层析架上,由biologic lp 系统调控。人工进样阀mv–6 进样阀有6 个带有凹luer 接头的口。它能与任何容积的自制上样环匹配。mv–6 阀直接安置于biologic 系统层析架和econo 梯度泵支架上。组分收集/层析柱旁通阀sv–3 分流/旁通阀是由econo 梯度泵、ep–1 型econo 泵或biologic lp 系统控制的二相螺线管阀。当与 biologic lp 系统连接时,sv–3 阀将液体从层析柱导入 至旁通位点,或至组分收集器或至废液位点。阀的功 能由后置的mini–din 接头和阀管线决定。当与 econo 泵或econo 梯度泵连接,sv–3 阀只作为组分收集器分流 阀使用。sv–3 阀直接与biologic 系统层析架和econo 梯度泵支架连接。 econo 梯度组合泵*的econo 梯度泵适用于任何低压蛋白纯化系统。它是*能独立应用于均一洗脱和 梯度洗脱的蠕动泵。econo 梯度泵是一个双通道双向泵,应用于制备层析或重组蛋白亲和 层析纯化。econo 梯度泵可与2110 型、biofrac 组分收集器,em–1 型econo 紫外检 测器、eg–1 型econo 梯度检测器和1327 型记录仪等设备*匹配。该econo 梯度 组合泵是您经济而实用的*。开发新的应用领域• 串联收集— 将层析柱流出液分流,收集至两个不同部位的试管或微孔板中,作进一部的测试和分析 • 取样操作— 发酵罐在发酵进程中可例行取样,以监控目标产品的浓度;培养液通过econo 梯度泵再循环,分流阀定时将等量试样送至组分收集器作进一步分析 • 实时生物活性检测— 流出液中添加底物来检测反应混合物。econo 梯度泵可提供一定流速的底物,并通过三 通管与分装的流出液混合。收集反应混合液作进一步 分析,或送至流动池记录生色团的变化 梯度泵具有以下优点:• 每个通道的流速0.002–40ml/min (每泵2 个通道,zui高流速达20ml/min );分装阀组合套件流速可达> 2μl/min • 控制二元梯度混合器 • 安装、操作简便 • 时间和体积两种梯度控制模式 • 控制分流阀收集废物或组分(尤其适合较大体积的分子量排阻层析) • 控制组分收集器的启动/停止;一些组分收集器具有“等待”和“暂停”功能 • 控制记录仪的启动/停止,记录笔的起/落 • 控制分装阀的百分比增量 • 根据不同规格管线自动调节收集程序 • 经济、简便、快速连接的luer–lok 接头 • 液晶显示器对比度可调,*的记数器确认管线磨损程度,有效按键蜂鸣声 • 节省空间的整合设计 • 与biologic duoflow 层析系统兼容 关于econo 梯度泵的更多信息,请索取资料# 2437 和# 2438。 bio–rad 公司向用户提供一系列全套的层析系统,这些产品秉 承了bio–rad 对用户的一贯承诺:即提供质优、灵活、可升级和使 用方便的产品。层析系统包括econo 蠕动泵和检测器等低压组件, 以及广受用户欢迎的biologic lp 低压系统,biologic duoflow 高 分辨率层析系统可方便地增添新组件而进行升级,方便用户定制自 己的系统。biofrac 组分收集器功能强大,为当今的层析研究提供 了诸多的灵活性;biologic maximizer 阀系统为biologic duoflow 层析系统提供了溶液自动配制及更高的流速。biologic duoflow 层析系统不但可灵活地与多种系统相匹配,而且提供了多种升级的选择,并有一个直观、易操作的通用控制软件。根据需要为系统升级以增添功能biologic duoflow 模块式的组件让用户可以根据实验室空间和使用需要,设计配置自己的生物层析系统。它 们可以用在实验工作台上或冷库中, 当实验要求改变时,将系统简单地重组并升级以增添新的功能,如提高流速、 完善检测功能、ph 监控、层析图比较和缓冲液自动配制等功能。biologic duoflow pathfinder 层析系统(图中所示包括附加的avr9–8 和sv5–4 阀),可简便地应用于多种层析技术操作,包括基本实验步 骤操作以及新方法新工艺的优化。biologic duoflow 系统选择指南系统组件所在的页 106 106 124 110 109 113 106–107biologic duoflow 基础系统3,500 psi 10 ml/min 流速254/280 nm 波长检测 标准 可升级 可升级 可升级 可升级 可升级 可升级biologic duoflow 标准系统3,500 psi 10 ml/min 流速254/280 nm 波长检测组分收集器 标准 可升级 标准 可升级 可升级 可升级 可升级biologic duoflow quadtec 基础系统3,500 psi 10 ml/min 流速4 波长同步检测 标准 可升级 可升级 标准 可升级 可升级 -biologic duoflow quadtec 标准系统3,500 psi 10 ml/min 流速4 波长同步检测组分收集器 标准 可升级 标准 标准 可升级 可升级 -biologic duoflow maximizer 20 系统3,500 psi 20 ml/min 流速254/280 nm 波长检测缓冲液配制ph 检测组分收集器 标准 可升级 标准 可升级 标准 标准 可升级biologic duoflow maximizer 80 系统1,000 psi 80 ml/min 流速254/280 nm 波长检测缓冲液配制ph 检测组分收集器 可升级 标准 标准 可升级 标准 标准 可升级biologic duoflow pathfinder 20 系统3,500 psi 20 ml/min 流速4 波长同步检测缓冲液配制ph 检测组分收集器 标准 可升级 标准 标准 标准 标准 -biologic duoflow pathfinder 80 system1,000 psi 80 ml/min 流速4 波长同步检测缓冲液配制ph 检测组分收集器 可升级 标准 标准 标准 标准 标准 -更多信息,见106-125 页,或索取资料# 2687a。bio–rad 公司为biologic duoflow 层析系统及其外围设备, 如用于缓冲液自动配制的biologic maximizer 阀系统、biologic quadtec 紫外/可见光检测器和biofrac 组分收集器等,提供操作合格性检验规程和服务。bio–rad 公司的iq/oq 操作规程是为帮助用户遵循美国fda 认证而设计的,由经过培训、并有资格的技术人员遵循nist 标准来完成。iq 服务确保biologic duoflow 层析系统正确安装,oq 服务确保所安装层析系统的性能并提供相应的安全特性记录。