RD 3339-FB-025R&D供应商Recombinant Rat FGF basic 重组大鼠碱性成纤维细胞生长因子
品牌
经销商厂商性质
上海市所在地
Hyclone*SH30042.01*0.25%
面议BIO-RAD伯乐8联管TLS0851
面议bio-rad八联管PCRTLS0801
面议ABI 384孔板荧光定量PCR用4309849
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面议sciencell MelM-2黑色素细胞培养基
面议sciencell FM成纤维细胞培养基
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面议GIBCO热灭活胎牛血清FBS,heat inactivated
面议GIBCO间充质干细胞MSC胎牛血清
面议sigma胶原酶2型(Collagenase,Type 2)
面议DESCRIPTION
Source E. coliderived
Ala11Ser154
Accession # P13109
Nterminal
Sequence
Analysis
Ala11
Predicted Molecular
Mass
16.2 kDa
SPECIFICATIONS
Activity Measured in a cell proliferation assay using NR6R-3T3 mouse fibroblast cells. Rizzino, A. et al. (1988) Cancer Res. 48:4266? Thomas, K. et
al. (1987) Methods Enzymol. 147:120.
The ED50 for this effect is typically 0.31.8
ng/mL.
Endotoxin Level <1.0 EU per 1 μg of the protein by the LAL method.
Purity >95%, by SDSPAGE
under reducing conditions and visualized by silver stain.
Formulation Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein. See Certificate of Analysis for
details.
PREPARATION AND STORAGE
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freezethaw
cycles.
l 12 months from date of receipt, 20
to 70
°C as supplied.
l 1 month, 2 to 8 °C under sterile conditions after reconstitution.
l 3 months, 20
to 70
°C under sterile conditions after reconstitution.
BACKGROUND
FGF basic (FGF2,
HBGF2)
is one of at least 22 mitogenic proteins of the FGF family, which show 35 60%
amino acid conservation (1 3).
Unlike other FGFs,
FGF acidic and basic lack signal peptides and are secreted by an alternate pathway. Storage pools within the cell or on cell surface heparan sulfate proteoglycans
(HSPG) are likely. The predicted 18 kDa FGF basic isoform can be located in both the cytoplasm and the nucleus and is presumed to be the form secreted (4).
Transcription from alternate start sites produces 21 23
kDa forms found only in the nucleus. High and low molecular weight human FGF basic targets the expression
of different genes when expressed in NIH3T3
cells (5). The 18 kDa rat sequence has 98% amino acid (aa) identity with mouse, and 96 97%
aa identity with human,
bovine and sheep FGF basic (6, 7). Autocrine, intracrine and paracrine actions of FGF basic have been identified. Binding of FGF to heparin or cell surface HSPG is
necessary for binding, dimerization and activation of tyrosine kinase FGF receptors. FGF basic binds other proteins, polysaccharides and lipids with lower affinity (3).
Expression of FGF basic is nearly ubiquitous but disruption of the mouse FGF basic gene gives a relatively mild phenotype, suggesting compensation by other FGF
family members. FGF basic modulates such normal processes as angiogenesis, wound healing and tissue repair, embryonic development and differentiation, neuronal
function and neural degeneration. Transgenic overexpression of FGF basic results in excessive proliferation and angiogenesis reminiscent of a variety of pathological
conditions (1 3).
References:
1. Coulier, F. et al. (1997) J. Mol. Evol. 44:43.
2. Fernig, D. et al. (1994) Prog. Growth Factor Res. 5:353.
3. Presta, M. et al. (2005) Cytokine Growth Factor Rev. 16:159.
4. Claus, P. et al. (2003) J. Biol. Chem. 278:479.
5. Quarto, N. et al. (2005) Gene 356:49.
6. Tsuneto, M. et al. (2005) Biochem. Biophys. Res. Commun. 335:1239.
7. Shimasaki, S. et al. (1988) Biochem. Biophys. Res. Commun. 157:256.
Recombinant Rat FGF basic
Catalog Number: 3339-FB
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