RD 1887-ml-010-RD白介23细胞因子Recombinant Mouse IL-23-,IL-23上海力敏实业有限公司手机版

详细介绍

Source:Spodoptera frugiperda, Sf 21 （stably transfected）-derived

Accession #:NP_072133 （p40） & NP_569094 （p19）

N-terminal Sequence Analysis:Met23

Purity:>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level:< 1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass:57 kDa

SDS-PAGE:66 kDa, reducing conditions

Activity:Measured by its ability to induce IL-17 secretion by mouse splenocytes. Aggarwal, S. et al. （2003） J. Biol. Chem. 278:1910.
The ED₅₀ for this effect is typically 0.05 - 0.25 ng/mL.

Protein Construct:

Mouse IL-23 p40 （Met23 - Ser335） Accession # P43432	IGSGSSRGGSGSGGSGGGGSK	Mouse IL-23 p19 （Leu20 - Ala196） Accession # Q9EQ14
N-terminus		C-terminus

What does CF mean?

CF stands for Carrier Free （CF）. We typically add Bovine Serum Albumin （BSA） as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increase shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

Which formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1887-ML	1887-ML/CF
Formulation: Lyophilized from a 0.2 µm filtered solution in PBS with BSA as a carrier protein. See Certificate of Analysis for details.	Formulation: Supplied as a 0.2 µm filtered solution in PBS. See Certificate of Analysis for details.
Reconstitution: Reconstitute at 10 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.	Reconstitution:
Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediay at the temperature recommended below.	Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediay at the temperature recommended below.
Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.	Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

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