CCR2/CKR2 antibody RabMAb® Cat.#: 2068-1
Abcam ID: ab32144

Rabbit Monoclonal Antibody

Download datasheet [PDF]|Related Products

Bulk and Custom formulation available request

Description

CCR2, C-C chemokine receptor type 2, is a G protein-coupled, seven transmembrane domain receptor protein. It has been found to be a monocyte chemoattractant protein 1 （MCP-1）-specific receptor （1,2）. Activation of the CCR2b isoform by MCP-1 binding has been shown to trigger intracellular Ca2+ flux and activation of the Jak/Stat pathway （3）. A variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors （4）, making elucidating their specific functions important for more fully describing the process of HIV infection.

Recommended Applications

IHC, IP, FC

Applications and Recommended Dilution Factors

IHC: 1:500 - 1:1,000
IP: 1:50
FC: 1:25
 

Species Reactivity *

Human, Mouse

*Species cross-reactivity is based on IHC analysis.

Products Data

Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CCR2/CKR2 RabMAb （catalog #2068-1）.	Flow cytometric analysis of permeabilized Jurkat cells using anti-CCR2/CKR2 RabMAb （red） （catalog #2068-1） or a rabbit IgG （negative） （green）.	Western blot analysis of immunoprecipitation pellets using （1） Jurkat cell lysate or （2） 1X PBS （negative control）, in the presence of anti-CCR2/CKR2 RabMAb （catalog #2068-1）.

Specificity

A synthetic peptide corresponding to the N-terminal residues of the human CCR2/CKR2 was used as immunogen. The antibody does not cross-react with other CKR family members.

Storage Condition and Buffer

Store at -20 °C. Buffer: Antibody buffer, sodium azide, glycerol, and BSA. Stable for 12 months from date of receipt.

Alternative Names

CCR2 antibody, CC-CKR-2 antibody, CCR2A antibody, CCR2B antibody, CD192 antibody, CKR2 antibody, CKR2A antibody, CKR2B antibody, CMKBR2 antibody, FLJ78302 antibody, MCP-1-R antibody, MGC103828 antibody, MGC111760 antibody, MGC168006 antibody, C-C chemokine receptor type 2 antibody, Monocyte chemoattractant protein 1 receptor antibody

Description References

1. Charo, I.F., et al. Molecular cloning and functional expression of two monocyte chemoattractant protein 1 receptors reveals alternative splicing of the carboxyl-terminal tails. Proc Natl Acad Sci USA. 91:2752-6 （1994）
2. Wong, L.M., et al. Organization and differential expression of the human monocyte chemoattractant protein 1 receptor gene. Evidence for the role of the carboxyl-terminal tail in receptor trafficking. J Biol Chem. 272:1038 45 （1997）
3. Mellado, M., et al. The chemokine monocyte chemotactic protein 1 triggers Janus kinase 2 activation and tyrosine phosphorylation of the CCR2B receptor. J. Immunol. 161: 805-13 （1998）.
4. Doranz, B.J., et al. A Dual-Tropic Primary HIV-1 Isolate That Uses Fusin and the Beta-Chemokine Receptors CKR-5, CKR-3, and CKR-2b and Fusion Cofactors. Cell 85:1149-1158 （1996）.
 

Product References

1. Chun KR, Bae EM, Kim JK, et al, Suppression of the lipopolysaccharide-induced expression of MARCKS-related protein （MRP） affects transmigration in activated RAW264.7 cells ,Cellular Immunology 256 （2009） 92–98
[Application: FC]
2. Baba T, Nakamoto Y, Mukaida N, Crucial Contribution of Thymic Sirp+ Conventional Dendritic Cells to Central Tolerance against Blood-Borne Antigens in a CCR2-Dependent Manner ,J. Immunol. 183 （2009） 3053 - 3063
3. Tugizov S, Herrera R, Veluppillai P, et al, Epstein-Barr Virus （EBV）-Infected Monocytes Facilitate Dissemination of EBV within the Oral Mucosal Epithelium ,Journal of Virology 81 （2007） 5484–5496
4. Kanamaru Y, Pfirsch S, Aloulou M, et al, Inhibitory ITAM Signaling by Fc RI-FcR Chain Controls Multiple Activating Responses and Prevents Renal Inflammation ,Journal of Immunology 180 （2008） 2669–2678
[Application: WB]
5. Lee EY, Chung CH, Khoury CC, et al, The monocyte chemoattractant protein-1/CCR2 loop, inducible by TGF-β, increases podocyte motility and albumin permeability ,Am J Physiol Renal Physiol. 297 （2009） F85-F94
[Application: ICC]
6. Lee EY, Chung CH, Khoury CC, et al, The monocyte chemoattractant protein-1/CCR2 loop, inducible by TGF-β, increases podocyte motility and albumin permeability ,Am J Physiol Renal Physiol 297 （2009） F85 - F94
7. Burt D, Salvidio G, Tarabra E, et al, The Monocyte Chemoattractant Protein-1/Cognate CC Chemokine Receptor 2 System Affects Cell Motility in Cultured Human Podocytes ,Am J Pathol 171 （2007） 1789-1799
[Application: ICC]
8. Brambilla R, Persaud T, Hu XC, et al, Transgenic Inhibition of Astroglial NF-κB Improves Functional Outcome in Experimental Autoimmune Encephalomyelitis by Suppressing Chronic Central Nervous System Inflammation ,J Immunol 182 （2009） 2628–2640
[Application: IHC]
9. Lu PR, Li LB, Liu GQ, et al, Opposite Roles of CCR2 and CX3CR1 Macrophages in Alkali-Induced Corneal Neovascularization ,Cornea. 28 （2009） 562-569
[Application: IHC]
10. Knerlich-Lukoschus F, Juraschek M, Blomer U, et al, Force-Dependent Development of Neuropathic Central Pain and Time-Related CCL2/CCR2 Expression after Graded Spinal Cord Contusion Injuries of the Rat ,Journal of Neurotrauma 25 （2008） 427-448
[Application: ICC]
11. Tarabra E, Giunti S, Barutta F, et al, Effect of the Monocyte Chemoattractant Protein-1/CC Chemokine Receptor 2 System on Nephrin Expression in Streptozotocin-Treated Mice and Human Cultured Podocytes ,Diabetes58 （2009） 2109 - 2118
12. D’Mello C, Le T, Swain MG, Cerebral Microglia Recruit Monocytes into the Brain in Response to Tumor Necrosis Factor Signaling during Peripheral Organ Inflammation ,J. Neurosci. 29 （2009） 2089 - 2102
[Application: ICC]
13. Baba T, Nakamoto Y, Mukaida N, Crucial Contribution of Thymic Sirp+ Conventional Dendritic Cells to Central Tolerance against Blood-Borne Antigens in a CCR2-Dependent Manner ,J. Immunol. 183 （2009） 3053 - 3063
14. Tarabra E, Giunti S, Barutta F, et al, Effect of the Monocyte Chemoattractant Protein-1/CC Chemokine Receptor 2 System on Nephrin Expression in Streptozotocin-Treated Mice and Human Cultured Podocytes ,Diabetes58 （2009） 2109 - 2118
[Application: IHC]
15. Ali S, O'Boyle G, Hepplewhite P, et al, Therapy with Nonglycosaminoglycan-Binding Mutant CCL7: A Novel Strategy to Limit Allograft Inflammation ,Am J Transplant 10 （2010） 47-58
[Application: IHC]
16. Fu ES, Zhang YP, Sagen J, et al, Transgenic inhibition of glial NF-kappa B reduces pain behavior and inflammation ,Pain 148 （2010） 509-518
[Application: IHC]
17. Yao Y, Tsirka SE, The C Terminus of Mouse Monocyte Chemoattractant Protein 1 （MCP1） Mediates MCP1 Dimerization while Blocking Its Chemotactic Potency ,J Biol Chem 285 （2010） 31509-31516
 

Related Products

Product Name	Catalog #
Goat Anti-Rabbit IgG （H+L） HRP	3053-1
Goat Anti-Rabbit IgG polymerized HRP	3051-1
Cell Lysis Buffer RabMAb®	3500-1
Goat Anti-Rabbit IgG HRP Antibody	3056-1
Goat Anti-Rabbit IgG DyLight® 405	3063-1
Goat Anti-Rabbit IgG DyLight® 488	3064-1
Goat Anti-Rabbit IgG DyLight® 550	3065-1
Goat Anti-Rabbit IgG DyLight® 594	3066-1
Goat Anti-Rabbit IgG DyLight® 633	3067-1
Goat Anti-Rabbit IgG DyLight® 650	3068-1