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Applications and Uses
Choose Precision Plus Protein Dual Color standards for SDS-PAGE:
伯乐双染蛋白maker现货161-0374
More Information
Loading buffer:
30% (w/v) glycerol, 2% SDS, 62.5 mM Tris, pH 6.8, 50 mM DTT, 5 mM EDTA, 0.02% NaN3, 0.01% bromophenol blue
MW size range, kD | 10–250 | |
Stain type | Dual Color | |
Volume, µl | 500 | |
Total number of applications | 50 | |
Shelf life at –20°C, months | 12 | |
Loading volume on mini gels, µl | for Coomassie staining, 10 | |
Loading volume on mini gels, µl | for fluorescent blot detection, 5 of 1:10 dilution | |
Application Type | ||
Monitoring electrophoretic separation for SDS-PAGE gels | Yes | |
Molecular weight sizing on SDS-PAGE gels | Yes | |
Monitoring blot transfer efficiency | Yes | |
Fluorescence blots | Yes | |
Molecular weight sizing on | N/A | |
Loading Volume on Mini Gels | ||
For Coomassie staining, µl | N/A | |
For stain-free gels, µ | N/A | |
For silver staining, µl | N/A | |
For SYPRO Ruby staining, µl | N/A | |
For blot development, µl | N/A | |
StrepTactin conjugate working dilutions for colorimetric blot development | N/A | |
StrepTactin conjugate working dilutions for chemiluminescence blot development | N/A |
The Precision Plus Protein™ Dual Color standards are a mixture of ten recombinant proteins (10–250 kD), including eight blue-stained bands and two pink reference bands (25 nd 75 kD).